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Alyson Mitchell Associate Professor and Food Chemist |
| Vice-chair of the Functional Foods & Natural Products Subdivision of Agricultural and Food Chemistry Division of the American Chemical Society | |
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| Contact Information: | |||
| Department of Food Science and Technology | Email: aemitchell@ucdavis.edu |
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| University of California | Office Phone: (530) 752-7926 | ||
| One Shields Avenue | Lab Phone: (530) 752-5095 | ||
| Davis, CA 95616-8598, USA | Office: FSTB 206 | ||
| Phone: (530) 752-1465 | Map and directions to Food Science Bldg | ||
| Recent Presentations/Activities: |
| A Comparison of the Nutrient and Phytochemical Content of Organic and Conventional Tomatoes and Peppers |
| Organic Seed Alliance, Portland, OR, 2006 |
| Identification of Glutathione Conjugates of Quercetin |
| 2nd International Conference on Polyphenols and Health, Davis, CA 2005 |
| Organizing Committee Member |
| 229th ACS National Meeting, San Diego, CA |
| Organizer, 2005 |
| Comparisons of Organic and Conventional Grown Tomatoes and Peppers |
| EccoFarm, 2005 |
| Metabolic Profiling of Quercetin Metabolites: Biomarkers of Phase II Metabolism |
| CIFAR, Davis, CA2004 |
| Sampling Issues Associated with Antioxidant Measurements in Food Crops |
| First International Conference on Antioxidant Methods, 2004 |
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| Laboratory Capabilities & Analyses: | ||
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Instrumental Capabilities: |
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Micromass triple quadrupole mass spectrometer interfaced to a Shimadzu LC system. | |
| Waters 2690 Alliance HPLC System equipped with a Waters 996 photodiode array detector and a Waters 474 scanning fluorescence detector. | ||
| HP 1090 HPLC system with PDA and flourescence detection (x2). | ||
| Shimadzu UV-1700 PharmaSpec spectrophotometer. | ||
| Integrated ESA electrochemical detector and workstation. | ||
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Phytochemical and Antioxidant Analyses: |
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| Proanthocyanidins - | ||
| Extraction and normal-phase HPLC separations of proanthocyanidin by degree of polymerization, MS characterization and quantitation by fluorescence. | ||
| Flavonoids - | ||
| Extraction and LC/MS/MS characterization of flavonoids and flavonoid gycosides in foods and biological tissues. | ||
| Generation and characterization of flavonoid metabolites in biological tissues. | ||
| Total Phenolics - | ||
| Spectrophotometric measurements of total phenolics in plant tissues. | ||
| Vitamins - | ||
| Extraction and HPLC analysis of ascorbic acid (vitamin C). | ||
| Lycopene determination by LC/MS. | ||
| Alkaloids - | ||
| Caffeine determination HPLC. | ||
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Biochemical Analyses: |
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| Purification of glutathione S-transferases mGSTA3, mGSTP1, mGSTM1. | |
| Spectrophotometric analysis of glutathione S-transferase, glucuronyl transferase and sulfotransferase activities. | |
| LC/MS/MS characterization of protein and peptides and assessment of protein modifications. | |
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